Edtech.com's Summary

Oregon Health & Science University is hiring a Research Assistant 2 or Senior Research Assistant. The role involves executing complex organoid experiments, developing functional assays, and managing multiple concurrent projects focused on intestinal development, nutrient uptake, and metabolism using patient-derived samples and mouse models. The position also includes assisting with molecular biology techniques, immunostaining, imaging, and animal handling within the Department of Pediatrics' research lab.

Highlights

Execute organoid experiments and develop assays to study human milk effects on intestinal development.
Generate neonatal patient-derived intestinal organoids and maintain sterile conditions.
Isolate human milk extracellular vesicles and perform analyses such as qPCR, western blotting, and microRNA sequencing.
Assist with neonatal mouse pup experiments, oral gavage, hypoxic chamber use, and tissue processing.
Experience required in wet bench laboratory work, cell culture, tissue handling, and molecular biology techniques.
Preferred qualifications include a Bachelor's degree in biological sciences, animal handling, organoid culture, microscopy, and bioinformatics.
Ability to manage multiple tasks independently and as part of a team with strong communication and organizational skills.
Position requires occasional evening and weekend work.
Salary is commensurate with experience, education, and internal equity.
Work conducted within the Department of Pediatrics at Oregon Health & Science University.

Research Assistant 2 -OR-Senior Research Assistant Full Description

Department Overview:

Sarah Andres' Lab is located within the Department of Pediatrics. The Andres Lab investigates intestinal epithelial differentiation, nutrient handling, and barrier function with a focus on the neonatal inflammatory disease necrotizing enterocolitis (NEC) and the use of human milk extracellular vesicles as a potential therapeutic. By using rare human patient samples, genetic mouse models, and cutting-edge patient-derived organoids, the Andres Lab is at the forefront of research in these fields evidenced by high impact publications and multiple funding sources.

We are currently recruiting for a Research Assistant 2 -OR-Senior Research Assistant depending on qualifications. Function/Duties of Position:

The Research Assistant is responsible for executing complex organoid experiments and helping to develop functional assays to delineate mechanisms of human milk action on intestinal development and maturation. This involves generating neonatal patient-derived intestinal organoids (enteroids) from surgical tissue samples. Cultivating enteroids requires keen attention to detail and impecable sterile technique. The Research Assistant is also responsible for isolating human milk extracellular vesicles via density-gradient ultracentrifugation, quantifying by Nanoparticle Tracking analysis, and running various downstream analyses or experiments including, qPCR, western blotting, immunostaining, imaging and cell uptake studies, microRNA sequencing, proteomics, and oral gavage into rodant models. Experiments are currently focused on nutrient uptake, lipid handing, and metabolism. The ability to manage multiple co-occuring experiments involving enteroids and/or mice is important. In addition to primary project responsibilitoes, this position will assist lab team members with experiments involving neonatal mouse pups, oral gavage, and use of a hypoxic chamber. This also includes intestinal tissue harvest and procesing, enteroid generation and propogation, immune cell isolation and culture, immunostaining and microscopy, and molecular biology.

In addition to research skills training, our team prioritizes mentorship and training in career development. Required Qualifications:

Required qualifications for Research Assistant 2:

Bachelor's in relevant field OR Associate's AND 3 years of relevant experience OR 4 years of relevant experience OR Equivalent combination of training and experience.
1+ years in a wet bench laboratory.

Required qualifications for Senior Research Assistant:

Bachelor's in relevant field AND 1 years of relevant experience OR Equivalent combination of training and experience.
Wet bench laboratory experience.

Job Related Knowledge, Skills and Abilities:

Working as part of a team
Working independently
Working in dynamic, fast paced environment
Ability to juggle multiple tasks
Ability to master detail-oriented techniques and procedures
Computer skills
Must have excellent communication, analytical and organizational skills: both written and verbal
Experience in cell culture
Experience in tissue handling
Experience in molecular biology techniques
Ability to multi-task
Flexible
Strong problem-solving skills

Preferred Qualifications:

Bachelor’s degree in biologic sciences
3+ years of Wet bench laboratory experience
Basic laboratory experience
Animal handling experience
Laboratory/cell/organoid culture experience
Cell culture techniques
Molecular biology skills
Bioinformatic experience Extracellular vesicle biology
Metabolism or metabolic studies experience
Microscopy
Immunostaining
Mouse handling experience

Additional Details:
***Upload cover letter and resume. Please be sure to include months and years (MM/YYYY format) to the resume for jobs/experience.
All are welcome: Oregon Health & Science University values a diverse and culturally competent workforce. We are proud of our commitment to being an equal opportunity, affirmative action organization that does not discriminate against applicants on the basis of any protected class status, including disability status and protected veteran status. Individuals with diverse backgrounds and those who promote diversity and a culture of inclusion are encouraged to apply. To request reasonable accommodation contact the Affirmative Action and Equal Opportunity Department at 503-494-5148 or aaeo@ohsu.edu.

Original Job Description

Department Overview:

We are currently recruiting for a Research Assistant 2 -OR-Senior Research Assistant depending on qualifications. Function/Duties of Position:

In addition to research skills training, our team prioritizes mentorship and training in career development. Required Qualifications:

Required qualifications for Research Assistant 2:

Bachelor's in relevant field OR Associate's AND 3 years of relevant experience OR 4 years of relevant experience OR Equivalent combination of training and experience.
1+ years in a wet bench laboratory.

Required qualifications for Senior Research Assistant:

Bachelor's in relevant field AND 1 years of relevant experience OR Equivalent combination of training and experience.
Wet bench laboratory experience.

Job Related Knowledge, Skills and Abilities:

Working as part of a team
Working independently
Working in dynamic, fast paced environment
Ability to juggle multiple tasks
Ability to master detail-oriented techniques and procedures
Computer skills
Must have excellent communication, analytical and organizational skills: both written and verbal
Experience in cell culture
Experience in tissue handling
Experience in molecular biology techniques
Ability to multi-task
Flexible
Strong problem-solving skills

Preferred Qualifications:

Bachelor’s degree in biologic sciences
3+ years of Wet bench laboratory experience
Basic laboratory experience
Animal handling experience
Laboratory/cell/organoid culture experience
Cell culture techniques
Molecular biology skills
Bioinformatic experience Extracellular vesicle biology
Metabolism or metabolic studies experience
Microscopy
Immunostaining
Mouse handling experience

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Research Assistant 2 -OR-Senior Research Assistant

Edtech.com's Summary

Research Assistant 2 -OR-Senior Research Assistant Full Description

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